PerfectStart® Uni RT-qPCR Kit is a two-step fluorescence quantitative PCR reagent known for its high synthesis efficiency and
amplification efficiency. In the same reaction system, it efficiently synthesizes the first-strand cDNA while simultaneously
removing genomic DNA, utilizing 5×All-in-One Reaction Mix for qPCR and TransScript® Uni All-in-One Enzyme Mix at
temperatures ranging from 42°C to 65°C. Additionally, it comes with 20×No RT Control Mix, to prepare a control without reverse
transcriptase, allowing the determination of whether the qPCR template originates from cDNA. The qPCR amplification is
performed using PerfectStart® Green qPCR SuperMix.
Features
• Efficient synthesis of first-strand cDNA from RNA templates using 5×All-in-One Reaction Mix for qPCR and TransScript® Uni
All-in-One Enzyme Mix, requiring only 5 minutes for reverse transcription. Simultaneously, genomic DNA is removed, ensuring
simplicity of operation protocol and reducing the risk of contamination during the process.
• The qPCR amplification is conducted using PerfectStart® Green qPCR SuperMix, known for its high amplification efficiency,
high specificity, excellent sensitivity and data accuracy.
• The kit comes with Universal Passive Reference Dye suitable for different instrument models. The Universal Passive Reference
Dye can adjust the variations between tubes caused by PCR pipetting errors, thereby ensuring data accuracy.
Applications
• High copy, low copy gene detection.
• RNA templates with high GC content or complex secondary structures.
Storage
at -20˚C in the dark for two years
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
