Real time PCR based analysis combines “traditional” end-point detection PCR with fluorescent detection technologies to record the accumulation of amplicons in “real time” during each cycle of the PCR amplification. Based on the advantages that it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput, real time PCR is among the most valuable techniques applied in biological researches, which comprise basic science, biotechnology, medicine, forensic science, diagnostics and more.
Owing to years of continuing innovation and improvement, TransGen has built abundant experience on real time PCR/ RT-PCR technologies combining reverse transcription and DNA amplification, based on which, comprehensive real time PCR/ RT-PCR solutions can be provided to push forward researchers’ experiments.
Click to find the whole list of Real Time PCR/ RT-PCR products
Multiple Premium RT Enzymes Converting RNA to cDNA in Real Time PCR/ RT-PCR
· Long transcripts of up to 20 kb due to reduced RNase H activity.
· High thermostability, active at temperatures from 42℃ to 65℃, ideal for low-abundance and complex RNA templates.
· Fast reaction: as short as 5 minutes.
PerfectStart Series: Triple Antibody Blocking-based Hot Start Polymerase for Real Time PCR/ RT-PCR
Fig. 1 Principle of triple antibody blocking-based hot start technology
Greater specificity
Fig. 2 58 genes (9 human genes, 7 mouse genes, 17 rice genes, 8 tobacco genes, 4 Arabidopsis genes, 10 wheat genes and 3 maize genes) were amplified using products from TransGen, Company V, and Company T. The highest number of genes with no NTC amplification was obtained by TransGen's PerfectStart™ Green qPCR Supermix (AQ601).
High amplification efficiency
Fig. 3 The amplification plot (left) and standard curve (right) resulting from the amplification of a dilution series of plasmid DNA (10 ng to 0.1 pg, 10-fold dilution) using PerfectStart™ Green qPCR SuperMix (AQ601). The amplification efficiency was 94.1%.
High sensitivity
Fig. 4 β-actin was amplified from a dilution series of human cDNA obtained by reverse transcription (TransGen, AT311) from 500 ng of RNA (no NTC amplification), using the products from TransGen and Company T respectively. The results show that amplification sensitivity of TransGen's PerfectStart™ Green qPCR Supermix (AQ601) is comparable to the product from Company T.
Superior Performance of Real Time PCR/ RT-PCR Reagents
PerfectStart™ Green qPCR SuperMix (Cat. No. AQ601)
Amplification results for GAPDH, using PerfectStart™ Green qPCR SuperMix from TransGen and products from Company TG and Company V respectively, show that PerfectStart™ Green qPCR SuperMix lead to perfect S-shaped amplification curves and ideal melting curves with one sharp peak. The reproducibility of PerfectStart™ Green qPCR SuperMix is comparable with Company TG’s reagent and better than Company V’s reagent.
Fig. 5 Amplification results of GAPDH using qPCR reagent kits from TransGen, Company TG and Company V, respectively.
PerfectStart™ II Probe qPCR SuperMix (Cat. No. AQ711)
PerfectStart™ II Probe qPCR SuperMix (Cat. No. AQ711) and the qPCR kit from Company TA are used for the amplification of DNA extracted from porcine serum infected with ASFV using EasyPure® Viral DNA/RNA Kit (Cat. No. ER201). The results for AQ711 and the qPCR kit from Company TA are comparable.
Fig. 6 Amplification results of DNA extracted from porcine serum infected with ASFV using qPCR reagent kits from TransGen and Company TA.
TransScript® II Probe One-Step qRT-PCR SuperMix (Cat. No. AQ321)
TransScript® II Probe One-Step qRT-PCR SuperMix (Cat. No. AQ321) and the qRT-PCR kit from Company TA are used for the detection of RNA extracted from cell culture medium infected with BVDV using EasyPure® Viral DNA/RNA Kit (Cat. No. ER201). The results for AQ321 and the qRT-PCR kit from Company TA are comparable.
Fig. 7 Amplification results of RNA extracted from cell culture medium infected with BVDV using qPCR reagent kits from TransGen and Company TA.
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG (Cat. No. AQ322)
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG (Cat. No. AQ322) is used for the detection of 2019-nCoV genes (ORF1ab, N) and human internal control gene (RNase P) from 2019-nCoV-specific in vitro-transcribed RNA standard diluted by HeLa total RNA. The result shows AQ322 enables multiplex amplification with a LOD of 500 Copies/ml.
Fig. 8 Amplification results of 2019-nCoV genes (ORF1ab, N) and human internal control gene (RNase P) from 2019-nCoV-specific in vitro-transcribed RNA standard diluted by HeLa total RNA using TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG (Cat. No. AQ322).
Real Time PCR/ RT-PCR Reagents Selection Chart
Click to learn about selection guide for Real Time PCR/ RT-PCR reagents
Highlighted Citations Related to Real Time PCR/ RT-PCR Reagents
1.Wu K, Wang S, Song W, et al. Enhanced sustainable green revolution yield via nitrogen-responsive chromatin modulation in rice. Science. 2020;367(6478):eaaz2046.
Cited products:
EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AE311)
TransStart® Green qPCR SuperMix（AQ101）
2.Li S, Tian Y, Wu K, et al. Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018;560(7720):595-600.
Cited products:
EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AE311)
TransStart® Green qPCR SuperMix（AQ101）
3.Xiang C, Du Y, Meng G, et al. Long-term functional maintenance of primary human hepatocytes in vitro. Science. 2019;364(6438):399-402.
Cited products:
TransScript® First-Strand cDNA Synthesis SuperMix （AT301）
4.Han X, Wang R, Zhou Y, et al. Mapping the Mouse Cell Atlas by Microwell-Seq. Cell. 2018;173(5):1307.
Cited products:
TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR （One-Step gDNA Removal）（AT341）
5.Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing. Nature. 2018;557(7706):585-589.
Cited products:
TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix（AT311）
6.Si W, Huang W, Zheng Y, et al. Dysfunction of the Reciprocal Feedback Loop between GATA3- and ZEB2-Nucleated Repression Programs Contributes to Breast Cancer Metastasis. Cancer Cell. 2015;27(6):822-836.
Cited products:
TransStart® Top Green qPCR SuperMix (AQ131)
7.Hongtai Z , Zhaogang S , Wenjing W , et al. Identification of Serum microRNA Biomarkers for Tuberculosis Using RNA-seq[J]. PLoS ONE, 2014, 9(2):e88909.
Cited products:
TransScript® Green Two-Step qRT-PCR SuperMix (AQ201)
8.Aqian L , Guifeng W , Yunfei W , et al. Identification of Intermediate-Size Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans[J]. PLoS ONE, 2012, 7(11):e48066.
Cited products:
TransScript® II Green One-Step qRT-PCR SuperMix (AQ311)
9.Wang P , Huang L , Jiang H , et al. Improving the Secretion of a Methyl Parathion Hydrolase in Pichia pastoris by Modifying Its N-Terminal Sequence[J]. PLOS ONE, 2014, 9(5):e96974.
Cited products:
TransStart® Probe qPCR SuperMix (AQ401)
Real Time PCR/ RT-PCR Reagents Related Pages
One-stop SARS-CoV-2 Detection Solutions One Stop from Viral Nucleic Acid Extraction to Detection
