TransDetect® Fluorescence DNase Detection Kit uses fluorescence-based method to rapidly and highly sensitively detect residual DNase in samples. Based on the principle of FRET: DNA probe tagged with a fluorescent reporter at the 5' end and a quencher at the 3' end is used as substrate. In the absence of DNase in sample, the quencher and reporter are near one another, and no fluorescent signal is generated; When DNase is present, the quencher and reporter are spatially separated in solution as DNA substrate is cleaved. This causes a fluorescence signal that can be detected. The DNA probe substrate has been optimized, which can detect as low as 10-3 U of DNase I and has good detection effect on various DNases, including DNase I, Exonuclease III, micrococcal nuclease, S1 nuclease, mung bean nuclease and Benzonase nuclease.
Applications
Suitable for detection of DNase in biological materials, experimental environments, reaction buffers, storage buffers, etc.
Storage
at -20℃ for two years.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
