TransDetect® Fluorescence RNase Detection Kit uses fluorescence-based method to rapidly and highly sensitively detect residual RNase in samples. Based on the principle of FRET: RNA probe tagged with a fluorescent reporter at the 5' end and a quencher at the 3' end is used as substrate. In the absence of RNase in sample, the quencher and reporter are near one another, and no fluorescent signal is generated; When RNase is present, the quencher and reporter are spatially separated in solution as RNA substrate is cleaved. This causes a fluorescence signal that can be detected. The RNA probe substrate has been optimized, which can detect as low as 0.3 pg of RNase A and has good detection effect on various RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease and Benzonase.
Applications
Suitable for detection of RNase in biological materials, experimental environments, reaction buffers, storage buffers, etc.
Storage
at -20℃ for two years.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
