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Cell Detection
  • FA301.jpg
FA301.jpg

TransDetect® In Situ Click TUNEL Imaging Kit-488 Fluorophore


Product Details

TransDetect® In Situ Click TUNEL Imaging Kit-488 Fluorophore is a TUNEL assay reagent based on the Click reaction. In apoptosis, double-strand breaks or single strand breaks of genomic DNA can produce a large number of sticky 3'-OH ends. EdUTP is a dUTP nucleotide modified with a small alkyne moiety, which is more readily incorporated at the 3´-OH ends of fragmented DNA by the Terminal deoxynucleotidyl transferase (TdT) enzyme. A covalent reaction occurs between the azide and the alkyne moiety on EdUTP based on the Click reaction, and more sensitive TUNEL detection can be performed via fluorophore on the azide.


Storage

 at -20˚C in the dark for one year


Shipping

Dry ice (-70℃)


Product Contents


ComponentFA301-01 (10 rxns)FA301-02 (50 rxns)
TdT Reaction Buffer-I (TRB-I)5 ml25 ml
EdUTP-I100 μl500 μl
TdT-I150 μl750 μl
Click Reaction Buffer 488 (CRB-488)5 ml25 ml
Reaction Buffer Additive (RBA)250 μl1.25 ml
Hoechst 3334250 μl250 μl
DNase I (3 units/μl)50 μl250 μl
10×DNase I Buffer500 μl2.5 ml


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