Steady-Luc Firefly Luciferase Reporter Assay Kit provides firefly luciferase reporter gene assay system with high-sensitivity, very stable and homogeneous fluorescence signal to measure quickly and efficiently intracellular luciferase expression without washing and collecting cells. The kit contains luciferin and optimized reaction reagents. This assay is based on luciferase system, the prepared Steady-Luc detection solution is directly added to the cell culture system to fully lyse the cells and release the luciferase, which can emit stable and detectable light signal.
The kit contains reaction buffer and substrate. Add an equal volume of the mixed Steady-Luc assay solution to the cell culture, and the signal can be detected in 3 minutes. The signal with high intensity and a half-life of more than 3 hours can be more suitable for high-throughput assays that have higher requirements for signal stability. It is easy to use, fast and efficient. And the signal is sensitive and stable.
Storage
At -20℃ in the dark for one year. The prepared Steady-Luc detection solution should be stored at -20℃ for 30 days, avoiding repeated freezing and thawing. If not used for a long time, it is recommended to store it at -80℃.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
